Processing
Archer™ VARIANTPlex™ Complete Solid Tumor panel
Identify DNA profiles of multiple cancers with one panel
Efficiently detect single nucleotide variants, copy number variations, insertions and deletions in 430 genes along with microsatellite instability and tumor mutational burden status with targeted NGS relevant for colorectal, breast, melanoma, gastric, pancreatic, CNS, NSCLC and other pan-cancer research.
Detect confidently with Archer VARIANTPlex NGS Panels for DNA.
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Learn how the VARIANTPlex Complete Solid Tumor panel can identify key genomic alterations for your research.
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Panel specifications
| Specifications | |
|---|---|
| Targeted genes | 430 |
| Genomic alterations | SNVs, Indels, CNVs, ITDs, MSI, TMB |
| Input nucleic acid required* | ≥10 ng |
| Recommended number of reads | 45 M |
| Hands-on time | <3.5 hours |
| Total library prep time | 1.5 days |
| Platform compatibility | Illumina® |
| Reagent format | Lyophilized or liquid |
| Supported sample types | FFPE, fresh frozen, cytology smear, FNA |
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.
Gene targets
AKT1
BRAF
EGFR
ERBB2
FOXL2
GNA11
GNAQ
GNAS
HRAS
IDH1
IDH2
KIT
KRAS
MET
NRAS
PDGFRA
PIK3CA
RET
TERT
TP53
ALK
APC
AR
ATRX
CDK4
CDK6
CDKN2A
CTNNB1
DDR2
ERBB3
ERBB4
ESR1
FBXW7
FGFR1
FGFR2
FGFR3
H3F3A
HIST1H3B
JAK2
MAP2K1
MAP2K2
MTOR
MYC
NOTCH1
NOTCH2
NOTCH3
NOTCH4
NTRK1
NTRK2
NTRK3
POLD1
POLE
PTEN
RAF1
RB1
RICTOR
ROS1
SMAD4
SMO
VHL
ATM
BARD1
BRCA1
BRCA2
BRIP1
CDK12
CHEK1
CHEK2
FANCA
FANCL
PALB2
RAD51B
RAD51C
RAD51D
RAD54L
STK11
ABL1
ACVR1
AKT2
AKT3
ARID1A
ARID1B
ARID2
ATR
AURKA
B2M
BAP1
BCOR
BLM
BMPR1A
CCND1
CCND2
CCND3
CCNE1
CDH1
CDKN2B
CHD1
CIC
CSF1R
DAXX
DDX3X
DICER1
EIF1AX
EP300
EPCAM
ERCC1
ERCC2
EZH2
FANCI
FGF19
FGFR4
FH
FLCN
FLT1
FLT3
FLT4
FOXA1
FUBP1
H3F3B
HIST1H3C
HNF1A
JAK1
JAK3
KDM6A
KDR
KEAP1
KLF4
KMT2C
KMT2D
LZTR1
MAP3K1
MDM2
MDM4
MED12
MEN1
MLH1
MPL
MRE11A
MSH2
MSH3
MSH6
MUC16
MUTYH
MYCN
NBN
NF1
NF2
NKX2-1
NPM1
PBRM1
PIK3CB
PIK3R1
PLCB4
PMS2
PPP2R1A
PPP2R2A
PRKD1
PTCH1
PTPN11
RAD50
RAD51
RHOA
RNF43
SDHA
SDHB
SDHC
SDHD
SETD2
SF3B1
SMAD2
SMARCA4
SMARCB1
SRC
SRSF2
STAG2
SUFU
TGFBR2
TP63
TRAF7
TSC1
TSC2
TSHR
U2AF1
XRCC2
XRCC3
ABL2
ACVR2A
ARAF
ARHGAP35
ARID5B
ASXL1
ASXL2
AURKB
AXIN1
AXIN2
AXL
BCL2
BCL2L1
BCL2L11
BCL6
BCORL1
BIRC3
BTG1
BTG2
BTK
CACNA1A
CARD11
CASP8
CBFB
CBL
CD274
CD70
CD79A
CD79B
CDC73
CDK8
CDKN1A
CDKN1B
CDKN2C
CEBPA
CHD3
CHD4
CHD8
COL5A1
CREBBP
CRKL
CSDE1
CSF3R
CTCF
CTLA4
CTNNA1
CUL3
CYSLTR2
DMD
DNMT3A
DOT1L
EEF1A1
EEF2
EGR3
ELF3
EPAS1
EPHA2
EPHA3
EPHA7
EPHB1
ERCC4
ERG
ERRFI1
EWSR1
FAM175A
FAM46C
FAM46D
FANCC
FANCD2
FANCE
FANCF
FANCG
FAT1
FGF1
FGF2
FGF3
FGF4
FGF7
FGF8
FGF9
FLNA
FOXA2
FOXO1
FOXP1
FOXQ1
GATA1
GATA2
GATA3
GEN1
GLI1
GNA13
GPS2
GRIN2D
GRM3
H3F3C
HGF
HIST1H1C
HIST1H1E
HIST1H2BD
HOXB13
HUWE1
IGF1R
IKBKE
IKZF1
IL6ST
IL7R
INPP4B
INPPL1
IRF2
IRF4
IRF6
IRS2
JUN
KANSL1
KDM5A
KDM5C
KEL
KIF1A
KLF5
KMT2A
KMT2B
KRT222
LAMP1
LATS1
LATS2
LEMD2
LRP1B
MACF1
MAP2K4
MAP3K13
MAP3K4
MAPK1
MAX
MCL1
MECOM
MEF2B
MGA
MGMT
MITF
MLLT3
MST1R
MUC6
MYCL
MYD88
MYH9
NCOR1
NFE2L2
NFKBIA
NIPBL
NSD1
NUP93
PARP1
PAX5
PAX8
PDCD1
PDCD1LG2
PDGFRB
PGR
PHF6
PIK3C2B
PIK3C2G
PIK3CD
PIK3CG
PIK3R2
PIK3R3
PIM1
PLCG1
PLXNB2
PMS1
POLQ
POLRMT
PPARG
PPM1D
PPP6C
PRDM1
PRKAR1A
PRKDC
PSIP1
PTMA
PTPDC1
PTPRC
PTPRD
PTPRT
RAC1
RAD21
RAD52
RARA
RASA1
RBM10
RECQL4
REL
RHEB
RIT1
RPL5
RPS6KB1
RPTOR
RRAS2
RUNX1
RUNX1T1
RXRA
SCAF4
SETBP1
SH2D1A
SLX4
SMAD3
SMC1A
SMC3
SOCS1
SOX17
SOX2
SOX9
SPEN
SPOP
SPTA1
SPTAN1
STAG1
STAT3
SYK
TAF1
TBL1XR1
TBX3
TCEB1
TCF3
TCF7L2
TET1
TET2
TFRC
TGFBR1
TGIF1
THRAP3
TLR4
TMSB4X
TNFAIP3
TNFRSF14
TRAF3
TXNIP
UNCX
USP9X
WT1
XPO1
ZBTB20
ZFHX3
ZMYM2
ZMYM3
ZNF750
Interested in adding a few genes to this panel?
Customize this NGS panel by adding any of our functionally-tested designs or create a new panel that fits your exact requirements with Assay Marketplace.
Benefits
- Detect confidently—A unique outlier detection algorithm leverages position-specific data to enable variant detection even at low allele frequencies, one of the many ways that Archer Analysis empowers you to detect confidently.
- Less QNS—Anchored Multiplex PCR (AMP™) chemistry is designed for compatibility with a wide range of sample types, including low-input and degraded samples such as FFPE tissue.
- Achieve efficiency—Streamlined workflows for your lab are enabled by choice of reaction-sized lyophilized reagents or high-throughput liquid reagents, while parallel workflows across all Archer panels provide efficient genomic characterization.
- Customize content—Use Assay Marketplace to modify any panel to fit your lab’s needs or start from scratch to keep up with the pace of discovery.
Ready to start?
Talk with our technical sales team. Learn how the VARIANTPlex Complete Solid Tumor panel can identify key genomic alterations for your research.
Request a consultationResources
Frequently asked questions
Can I analyze solid tumor DNA and RNA simultaneously for comprehensive genomic profiling with Archer panels?
Yes, DNA and RNA from a single sample can be analyzed with VARIANTPlex™ and FUSIONPlex™ panels to provide a genomic profile of the cancer. For comprehensive genomic profiling, pair VARIANTPlex Complete Solid Tumor and FUSIONPlex Pan Solid Tumor v2 panels to interrogate 511 genes for SNVs, indels, CNVs, fusions, exon-skipping, and splicing variants, as well as assessment of MSI and TMB. Additionally, the IMMUNOVerse™ TCR panel can provide tumor-infiltrating lymphocyte information relevant for solid tumor characterization. All Archer panels have parallel workflows, allowing for streamlined comprehensive solid tumor profiling.
Can VARIANTPlex™ assays be used for somatic and germline evaluation?
Most VARIANTPlex assays can be used for both somatic and germline variant detection. However, some custom‑designed panels for germline applications may not be suitable with FFPE tissue with shorter fragment lengths.
What is the difference between Anchored Multiplex PCR (AMP™) and traditional priming methods for DNA NGS analysis?
As opposed to traditional priming methods, AMP chemistry enables tiling primers across both strands of DNA, optimally covering targeted regions for amplification and characterization of challenging, yet relevant alterations such as internal tandem duplications. Additionally, AMP chemistry uses molecular barcode adapters that bind to DNA fragments before amplification, allowing for efficient amplification of targets even from degraded or fragmented nucleic acid input, such as DNA from FFPE tissue and ctDNA from plasma.
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