The Archer® Reveal ctDNA™ 28 Kit for Illumina® is an advanced and user-friendly solution for targeted next generation sequencing (NGS) of circulating cell-free tumor DNA (ccfDNA/cfDNA/ctDNA) from 28 genes commonly found mutated in solid tumor type cancers.
For Research Use Only. Not for use in diagnostic procedures.
# of GSP2s
Input cfDNA required
Recommended # of reads
Target bases covered
Raw molecular on-target
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The Archer Reveal ctDNA 28 Concordance Kit combines Archer VariantPlex® with Reveal ctDNA 28 for powerful copy number and variant detection from solid tumors and sensitive mutation detection from ctDNA, respectively. The kit also includes the Archer PreSeq® DNA QC Assay to prescreen DNA FFPE samples prior to library prep.
Concordance Kit Contents:
The Reveal ctDNA reagent kit preferentially amplifies highly fragmented circulating tumor DNA (ctDNA) over larger genomic fragments (i.e. lysed WBCs) that would otherwise compromise sensitive variant detection. Single-use lyophilized reactions in a convenient 1-day workflow requiring ~1 hour of total hands-on time dramatically simplifies complex NGS library preparation procedures, reduces the chance of operator error and improves overall inter-assay reproducibility.
Powerful and simple-to-use Archer Analysis software ensures sensitive and accurate mutation detection, complete data ownership and in-house data analysis, providing a useful alternative to send-out services that require high sample input volumes and have long turnaround times.
ctDNA comprises only a small fraction of cfDNA. Considering that driver mutations are often low-frequency mutations (allele fraction <2%), detecting such low-abundance mutations in highly fragmented material can be challenging. The Reveal ctDNA 28 kit detects relevant ctDNA variants at allele fractions (AF) down to at least 0.25% with varying input amounts, demonstrating high sensitivity and accurate low-AF variant detection.
The Archer Reveal ctDNA 28 kit detected 100% of variants in positive controls at 1% and 0.25% AF using 10 and 100ng inputs. Assays were performed in triplicate with 5M reads.
Average allele fractions across variants covered by the Archer Reveal ctDNA 28 kit from positive controls at 1% and 0.25% AF using 10 and 100ng inputs. Assays were performed in triplicate with 5M reads.
Low-frequency mutation detection requires increased sequencing depth, which intrinsically results in more sequencing errors and greater noise in the data that leads to false-positive calls. In AMP chemisty, template DNA fragments are ligated to molecular barcodes, which the Archer Analysis bioinformatics platform uses for read provenance to correct for PCR and sequencing errors, thus reducing false-positive calls.
AMP is designed with simplicity in mind. The Reveal ctDNA 28 kit uses lyophilized reagents in single-use 8-tube strips for flexible run sizes and minimun cross-contamination. The workflow comprises serial transfers that total 1 hour of hands-on time for single-day library prep.
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