Archer target enrichment chemistry
Anchored Multiplex PCR target enrichment chemistry

At the heart of every Archer® assay is Anchored Multiplex PCR (AMP™), a target enrichment chemistry developed by molecular pathologists to create target-enriched libraries for next generation sequencing (NGS). AMP leverages the power of unidirectional gene-specific primers (GSPs), sample indexes and molecular barcodes for multiplex targeted NGS using low-input clinical sample types such as FFPE sections. AMP chemistry is flexible, so it can be used for applications in targeted RNA sequencing, genomic DNA sequencing and various genotyping applications.

Key features of AMP chemistry

Molecular barcodes (MBCs)
Adapters ligated to molecules before amplification carry MBCs that enable unique molecule counting and error correction for confident mutation calling
Strand-specific priming
Dual, independent coverage across target regions ensure that some reads are retained when one primer drops out due to primer-blocking SNVs
Unique start sites
Open-ended amplification gives flexibility for novel fusion detection in RNA and strand-specific bidirectional coverage in DNA
Targets can be added to assays to match the pace of discovery without negatively impacting assay performance
Low-input FFPE
AMP chemistry was purpose-built for highly fragmented low-yield sample types such as FFPE
Application flexibility
From cancer and germline diseases to immune repertoire and beyond. AMP chemistry can power new sequencing applications

Flexible input types

AMP chemistry is second to none when it comes to input type and application flexibility. AMP works well with both RNA and DNA inputs and is at the heart of all Archer NGS assays.

RNA chemistry

Archer FusionPlex chemistry

DNA chemistry

Archer VariantPlex chemistry

Why is AMP better for targeted NGS?

Error correction


Novel fusion detection


Quantitative unique reads


Strand-specific priming


NEXT: Learn about the Archer workflows, the simplest way to generate NGS libraries

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All content © 2019 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.